Description:

Welcome to our NGS: RNA-seq course where you will gain a comprehensive understanding of how to analyse and visualise bulk RNA-seq data.

Before taking this course, it is important to understand the basics of NGS by taking the NGS: Introduction and pre-processing pipeline course.

This course is for you if

• You have basic knowledge of R programming language which may have been obtain via our Pillar 2: Basic R with Data Carpentry course
• You work in a biomedical field
• You require flexible learning format
• You need to start using RNA-seq data
• You want to gain a better understanding of what information you can get from RNA-seq data
• You would like to learn how to carry out differential expression analysis for bulk data

Learning objectives

• Obtain a clear understanding of how RNA-seq data are analysed and visualised
• Understand how differentially expressed genes are detected
• Perform differential expression analysis test
• Use R to read in NGS data and annotate features